142 Mitochondrial abnormalities in the psoriatic uninvolved keratinocytes

In psoriasis, keratinocytes of the healthy-looking uninvolved skin are more sensitive to stress. Our previous study suggested that cell-stress proteins showing altered expression in psoriatic vs. healthy can affect mitochondrial functions. Therefore, we aimed examine whether involved these alterations, and compared functions between cultured detail by high–resolution respirometry. We did not find any significant difference oxidative phosphorylation capacity, ATP-independent respiration, residual oxygen consumption keratinocytes. However, maximal respiratory capacity was significantly lower Uninvolved showed a mild increase cytochrome c release cells. H2O2release, as marker reactive species, higher during leak respiration Moreover, observed stronger staining intensity immunolabeling frequent number cells intense conclusion, our results suggest increased biogenesis dysfunctions These alterations sensitivity stress provide further evidence about stressed state epidermis. Further studies may reveal changes have protective role or make susceptible for lesion formation.